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Figure 2 | BMC Medical Genetics

Figure 2

From: Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes

Figure 2

Tm analysis with SYBR® Green I of 101-bp PCR products to discriminate the H/R alleles. Panel A: Melting profile without DMSO. Panel B: Melting profile with DMSO 5%. Panel C: Melting profile with DMSO 10%. Heterozygotes are depicted as thick lines, the heterozygous pattern is distinguishable from the two homozygous curves because of the shoulder, most likely due to the presence of heteroduplexes.

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BMC Medical Genetics

ISSN: 1471-2350

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