Figure 1From: Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probesRelative positions of primer pair sets A and B designed to perform allele-specific PCR typing at the HA-1 locus. A(1) is the forward primer common to site-specific primers A(2) and A(3) designed with the 3'OH-end at c.500C/T. B(4) is the reverse primer common to site-specific primers B(5) and B(6) designed with the 3'OH-end at c.504A/G. Primer combinations A(1) + A(2) and B(4) + B(5) are specific for HA-1H. Primer combinations A(1) + A(3) and B(4) + B(6) are specific for HA-1R.Back to article page