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Table 1 Primers and conditions for PCR

From: Molecular breakpoint cloning and gene expression studies of a novel translocation t(4;15)(q27;q11.2) associated with Prader-Willi syndrome

RT-PCR
   Gene/Exon fwd (5'-3') rev (5'-3'); complement strand size T ann.
   ZNF127 GGG TTG CGG TTT TGC TAT TA TTT CTC GTG TGC TTC AAT GC 168 bp 59C
   MAGEL2 CTG AAG CCT GGG ACT TTC TG GGA CCT TGG CCA CAA ACT TA 225 bp 59C
   Necdin GAA GAA GCA CTC CAC CTT CG CCA TGA TTT GCA TCT TGG TG 164 bp 59C
   SNRPN Ex 1–3 ATG GAG CGG GCA AGG GAT CGC GGT ACA ACT GAC ACT CTT GG 124 bp 53C
   SNRPN Ex 14/16 CTG CAA ACA TAG GAG ATG ATA GTT CC CTT ATG AAA GCA CTG AGA TGA AGC C 459 bp 53C
   SNRPN Ex 16/17 GAA AGT GAC CTA AAG AGT GTC ATT G CTT GCA GTT GGA CAG CCG ACT C 515 bp 53C
   SNRPN Ex 17/18 AGA TAT CTT TAA AAT TGA GTC TTC TGT CCA TGA AGA TGC AGC ACT TTT GAA GAA 218 bp 53C
   SNRPN Ex 19/20a CAT TGT GCT TAT TTA CTA TTT TTG TAG ACG CTG CAG GTG GTG ACC ATG TG 150 bp 53C
   AK094315 TCT TCT CTA CCC TCA TTC CCA GC TCG CTA CAC CCC TTT GCT TAT G 222 bp 53C
   AB061718 AGG AGG GGT TCA AAG ATG C CTG GTA AAC AAA CTG GTA AAG GTG 204 bp 50C
   HBII-85/PWCR1 CGA TGA TGA GTC CCC CAT AAA AAC CAG TTC CGA TGA GAA CGA CG 79 bp 53C
   HBII-13 GGA TTT GTG ATG AGC TGT GTT TAC GGA CTT CAG AGT AAT CAC GTT G 67 bp 54C
   HBII-438A/B GGA TCG ATG ATG AGA ATA ATT ATT G GGA CCT CAG ATT GAC ATC TG 67 bp 53C
   GABRB3 TCA GGC GGC ATT GGC GAT ACC ATA AAA ACT TGA CAG GCA GAG 352 bp 52C
   GABRA5 AAT ATT GCC TTA ATG TTT CTA GCC TAT TCT ATT TCT TCG TGT 425 bp 48C
   GABRG3 GCG TAT TCA CAT AGA CAT CTT G GAT TGG TCA CTA CTG GTC TGG 188 bp 52C
   GAPDH TGG GCT ACA CTG AGC ACC AG GGG TGT CGC TGT TGA AGT CA 50 bp 53C
Quantitative RT-PCR
   Gene/Exon fwd (5'-3') rev (5'-3');complement strand size T ann.
   SNURF Ex2 ACG AAC TAC AGA ACA GCA CGT ACC CTG CGT TTG ACT TGG ACT TCC 50 bp 60C
   SNURF Ex3 TTC TCA GCA GCA GCA AGT ACC T TGC CTC AGT TCA GCC TGG A 50 bp 60C
   HBII-437 ATC ATT ATT TCT TGA ATT GG CCC TCA CGC TCC CTT TGC 50 bp 60C
   SNRPN Ex 14/15 CTG CAA ACA TAG GAG ATG ATA GTT CC CAA AGA CGA TAA AAT GTT CCT TCT TG 50 bp 60C
   SNRPN Ex 19/20a GGA ACC ACC ATT TGT CTA TGA TCC CTG CAG GTG GTG ACC ATG TG 50 bp 60C
   HBII-438 ATA ATT GTC TGA GGA TGC T GAT TGA CAT CTG GAA TGA GTC 50 bp 60C
   HBII-85/PWCR1 TCG ATG ATG AGT CCC CCA TAA CAT TTT GTT CAG CTT TTC CAA GG 50 bp 60C
PCR to generate Southern probes in intron 17
   Gene/Exon fwd (5'-3') rev (5'-3');complement strand size T ann.
   SB 1 ACC ATC AGT GAA TGA CCT GTT GC CCC AGC CTC TTT CCT ATG TCT TG 565 bp 53C
   SB 3 TGG TAA ACT GAT GAG AGC ACA GCC GCC TGG GAG ACA GAA TGA GAA AC 416 bp 53C
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