RT-PCR | ||||
---|---|---|---|---|
   Gene/Exon | fwd (5'-3') | rev (5'-3'); complement strand | size | T ann. |
   ZNF127 | GGG TTG CGG TTT TGC TAT TA | TTT CTC GTG TGC TTC AAT GC | 168 bp | 59C |
   MAGEL2 | CTG AAG CCT GGG ACT TTC TG | GGA CCT TGG CCA CAA ACT TA | 225 bp | 59C |
   Necdin | GAA GAA GCA CTC CAC CTT CG | CCA TGA TTT GCA TCT TGG TG | 164 bp | 59C |
   SNRPN Ex 1–3 | ATG GAG CGG GCA AGG GAT CGC | GGT ACA ACT GAC ACT CTT GG | 124 bp | 53C |
   SNRPN Ex 14/16 | CTG CAA ACA TAG GAG ATG ATA GTT CC | CTT ATG AAA GCA CTG AGA TGA AGC C | 459 bp | 53C |
   SNRPN Ex 16/17 | GAA AGT GAC CTA AAG AGT GTC ATT G | CTT GCA GTT GGA CAG CCG ACT C | 515 bp | 53C |
   SNRPN Ex 17/18 | AGA TAT CTT TAA AAT TGA GTC TTC TGT CCA | TGA AGA TGC AGC ACT TTT GAA GAA | 218 bp | 53C |
   SNRPN Ex 19/20a | CAT TGT GCT TAT TTA CTA TTT TTG TAG ACG | CTG CAG GTG GTG ACC ATG TG | 150 bp | 53C |
   AK094315 | TCT TCT CTA CCC TCA TTC CCA GC | TCG CTA CAC CCC TTT GCT TAT G | 222 bp | 53C |
   AB061718 | AGG AGG GGT TCA AAG ATG C | CTG GTA AAC AAA CTG GTA AAG GTG | 204 bp | 50C |
   HBII-85/PWCR1 | CGA TGA TGA GTC CCC CAT AAA AAC | CAG TTC CGA TGA GAA CGA CG | 79 bp | 53C |
   HBII-13 | GGA TTT GTG ATG AGC TGT GTT TAC | GGA CTT CAG AGT AAT CAC GTT G | 67 bp | 54C |
   HBII-438A/B | GGA TCG ATG ATG AGA ATA ATT ATT G | GGA CCT CAG ATT GAC ATC TG | 67 bp | 53C |
   GABRB3 | TCA GGC GGC ATT GGC GAT ACC | ATA AAA ACT TGA CAG GCA GAG | 352 bp | 52C |
   GABRA5 | AAT ATT GCC TTA ATG TTT CTA | GCC TAT TCT ATT TCT TCG TGT | 425 bp | 48C |
   GABRG3 | GCG TAT TCA CAT AGA CAT CTT G | GAT TGG TCA CTA CTG GTC TGG | 188 bp | 52C |
   GAPDH | TGG GCT ACA CTG AGC ACC AG | GGG TGT CGC TGT TGA AGT CA | 50 bp | 53C |
Quantitative RT-PCR | ||||
   Gene/Exon | fwd (5'-3') | rev (5'-3');complement strand | size | T ann. |
   SNURF Ex2 | ACG AAC TAC AGA ACA GCA CGT ACC | CTG CGT TTG ACT TGG ACT TCC | 50 bp | 60C |
   SNURF Ex3 | TTC TCA GCA GCA GCA AGT ACC T | TGC CTC AGT TCA GCC TGG A | 50 bp | 60C |
   HBII-437 | ATC ATT ATT TCT TGA ATT GG | CCC TCA CGC TCC CTT TGC | 50 bp | 60C |
   SNRPN Ex 14/15 | CTG CAA ACA TAG GAG ATG ATA GTT CC | CAA AGA CGA TAA AAT GTT CCT TCT TG | 50 bp | 60C |
   SNRPN Ex 19/20a | GGA ACC ACC ATT TGT CTA TGA TCC | CTG CAG GTG GTG ACC ATG TG | 50 bp | 60C |
   HBII-438 | ATA ATT GTC TGA GGA TGC T | GAT TGA CAT CTG GAA TGA GTC | 50 bp | 60C |
   HBII-85/PWCR1 | TCG ATG ATG AGT CCC CCA TAA | CAT TTT GTT CAG CTT TTC CAA GG | 50 bp | 60C |
PCR to generate Southern probes in intron 17 | ||||
   Gene/Exon | fwd (5'-3') | rev (5'-3');complement strand | size | T ann. |
   SB 1 | ACC ATC AGT GAA TGA CCT GTT GC | CCC AGC CTC TTT CCT ATG TCT TG | 565 bp | 53C |
   SB 3 | TGG TAA ACT GAT GAG AGC ACA GCC | GCC TGG GAG ACA GAA TGA GAA AC | 416 bp | 53C |