Molecular analysis using DHPLC of cystic fibrosis: increase of the mutation detection rate among the affected population in Central Italy

Table 1 Primers and DHPLC (oven temperature, gradient) analysis conditions for 6b and 9 exons of the CFTR gene

exon	Primer 5' → 3'	Amplicon length	Oven temp (°C)	% B buffer start/end
6b	F – CAGAGATCAGAGAGCTGGG	323	56	55/63
	R – GAGGTGGAAGTCTACCATGA
9	F – GGGATTTGGGGAATTATTTG	279	55	54/62
	R – TCTCCAAAAATACCTTCCAG

ISSN: 1471-2350