Figure 4

Analysis of RNA from CFBE41o^- (A, B) and 16HBE14o^- (C, D) transfected cells at different lipid/DNA ratios and harvested 120 hrs after transfection. Lanes 1-7 correspond to lipid/DNA charge ratios ranging from 42+/1- to 1+/1-. Lane 8 is RNA analysis of untransfected cells (A, C). The expression of the recombinant allele was detected in lanes 1 to 6 in (A) and in lane 6 only in (C). Densitometric analysis revealed that the maximum correction efficiency in CFBE41o^- is reached with a lipid/DNA ratio of 6+/1- (B), whereas in 16HBE14o^- recombinant allele is expressed only at 2.2/1 charge ratio (+/-) as reported in (D).