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Figure 2 | BMC Medical Genetics

Figure 2

From: A PCR-mutagenesis strategy for rapid detection of mutations in codon 634 of the retproto-oncogene related to MEN 2A.

Figure 2

Check of the integrity of codon 634 with the BstApI enzyme. DNA samples were amplified using the forward and reverse primers shown in figure 1. The PCR product was incubated in the presence (lane 1–4) or absence (lane 5) of BstApI. The restriction fragments were run in a non-denaturing 10% PAGE. Lane 1: mutation in the first base of the 634 codon (T>C); lane 2: mutation in the second base of the 634 codon (G>A); lane 3: mutation in the third base of the 634 codon (C>G); lane 4: wild type 634 codon; lane 5: wild type 634 codon without enzyme incubation. Both alleles are completely cut by the enzyme in the wildtype codon, which means that the codon is correct. In the rest of the samples, the product is not completely cut, which reveals that one allele is mutated at one of the three bases of codon 634.

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