Table 1 Primers and conditions used in PCR for ADAR1 gene
From: Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria
Exon | Primers (5′ → 3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
1 | Forward: ACTTCCAGTGCGGAGTAGCGGAG | 221 | 66 |
Reverse: CAAGACGCACACGCTACGCACTGC | |||
2 | Forward: TCAAGGGCTGTTCCACAGGCAGC | 1754 | 61 |
Reverse: TCAGCCAAGACTGCGTCAGGAGC | |||
3-4 | Forward: GGCAGAAGGAGTGACCTAGACTC | 907 | 66 |
Reverse: GGTCAATCTGCCATCCCTGAGGAGG | |||
5-6 | Forward: AGAGGCTAGGTCAGGCTCCTCAGTC | 674 | 66 |
Reverse: TAAGAGGCCGTGGAGACAGGGCT | |||
7-8 | Forward: CATATGTTTGCAAGACTGGCC | 835 | 63 |
Reverse: TGCATGGACTCCAGGGGAGGATGAG | |||
9 | Forward: CTGAGGCTGTTTCTGCCTTGAAGC | 253 | 63 |
Reverse: GGGAACTGGAGCTCTCCACAG | |||
10 | Forward: GCCCTCAGCAGAAATGAAGGAGACCC | 456 | 65 |
Reverse: CTCAAACCCACAGTGGAGTGTGGC | |||
11 | Forward: CTGCTGTCCACCTCCAGTCTCCCAT | 385 | 66 |
Reverse: CAGCACCTCTGTGCCCAGTGAC | |||
12 | Forward: GTGGTTTGGTCCATTGGCGCCTGTG | 300 | 66 |
Reverse: GCATGCAGTTTGGGATCTGGGCAC | |||
13-14 | Forward: CTGGTAGCCCCAGTCAGAAGGTGCT | 864 | 66 |
Reverse: GATGCACCCTTGCAAGTCAGGGCAG | |||
15 | Forward: ACGGTGTCTCCACTGTGAGCTCCT | 372 | 63 |
Reverse: GCTACGACCTACCTCTCTCACACCC |