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Figure 1 | BMC Medical Genetics

Figure 1

From: A simple method for gene phasing using mate pair sequencing

Figure 1

Mate pair library preparation. The MP protocol allows sequence information to be linked across greater distances than PE reads. Fragments of sheared DNA from a pool (500 – 5000 bp with an average of 2000 bp) are end-repaired using biotinylated nucleotides. Fragments are then self-ligated and all remaining linear DNA is removed by exonuclease treatment. Circularized DNA is fragmented again (black bars) to an average size of 500 bp and segments containing biotinylated junction points are isolated on streptavidin beads. In addition to fragments containing junction points, a portion of non-biotinylated DNA is co-purified and appears in the MP library as a subpopulation of PE reads. All fragments are end-repaired and indexed using TruSeq adapters followed by sequencing.

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