DNA methylation within the I.4 promoter region correlates with CYPl19A1 gene expression in human ex vivo mature omental and subcutaneous adipocytes

Table 1 Primer sequences for primers used in the PCR and pyrosequencing reactions

	Primer name	Sequence (5′ to 3′)
I.4.1, I.4.2^a	I.4a PCR F	GGAATGGTGAGAGTTTGGTTAATG
	I.4a PCR R	*CAAAAAACACCCTAAAATATAACCTACAA
	I.4a Seq.^b	GTAAAGTGTTTGTTTTTTTATAGT
I.4.3, I.4.4^a	I.4c PCR F	GGAATGGTGAGAGTTTGGTTAATG
	I.4c PCR R	*CAAAAAACACCCTAAAATATAACCTACAA
	I.4c Seq.^b	CTACAATAAAAACACATTTCTT
I.4.5	I.4b PCR F	*AGGAGATTTTTGATTTATGTGGGGTTATG
	I.4b PCR R	ACTCAAACTCCAAAAACTTACCTAAT
	I.4b Seq.^b	CCCATCACATCACTC

^aEncompasses two CpG sites upstream of the promoter. ^bSequencing primer. *Biotinylated at 5′ end and HPLC purified to enable capture of the PCR products by the PyroMark vacuum station filter probes.