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Table 2 Summary of products and their sizes after digestion with Fermentas Fast Digest® HinfI, Fermentas Fast Digest® MspI and NEB® BanII restriction enzymes

From: A novel multiplex PCR-RFLP method for simultaneous detection of the MTHFR 677 C > T, eNOS +894 G > T and - eNOS -786 T > C variants among Malaysian Malays

SNPs

Band size (bp)

Restriction enzyme

Restriction enzyme recognized sequences

MTHFR 677 C > T

 

Fermentas Fast Digest® HinfI

5′-GANTC-3′

 CC-Wild type

248

 CT-Heterozygote

248, 130, 118

 TT-Homozygote

130, 118

eNOS −786 T > C

 

Fermentas Fast Digest® MspI

5′-CCGG -3′

 CC-Wild type

178

 TC-Heterozygote

178, 137, 41

 TT-Homozygote

137, 41

eNOS +894 G > T

 

NEB® BanII

5′-G(G/A)GC(T/C)C -3′

 GG-Wild type

371

 GT-Heterozygote

371, 223, 139

 TT-Homozygote

223, 139