Table 1 Primers for MTHFR 677 C > T , eNOS +894 G > T and eNOS -786 T > C
Primer | Sequences | Length (bases) | Tm* (°C) | GC (%) | References |
|---|---|---|---|---|---|
677 F | 5′-CTC GCC TTG AAC AGG TGG AG-3′ | 20 | 58.3 | 60 | Self-designed |
677R | 5′-CTG GAT GGG AAA GAT CCC GG-3′ | 20 | 58 | 60 | Self-designed |
894 F | 5′-GTC CCT GAG GAG GGC ATG AG-3′ | 20 | 59.9 | 65 | [18] |
894R | 5′-TCC AGC AGC ATG TTG GAC AC-3′ | 20 | 58.2 | 55 | [18] |
786 F | 5′-GCA GGT CAG CAG AGA GAC TA-3′ | 20 | 55.9 | 55 | [19] |
786R | 5′-GAC ACA GAA CTA CAA ACC CC-3′ | 20 | 53.6 | 50 | [19] |