Figure 5
Comparison of I Ks -WT and I Ks -G60D channel currents at 37°C. KCNE1-WT (A) and KCNE1-G60D (B) channel subunits co-expressed with KV7.1 in CHO cells. Current protocol shown as inset. C: I/V relationship for IKs-WT (n = 15) and IKs-G60D (n = 12). Currents were measured at the end of the 2-second test pulse (open and filled squares) and normalized to cell size. D: Voltage-dependence of IKs-WT and IKs-G60D channel activation. Peak tail-currents were measured at -40 mV (open and filled circles), and the normalized data were fit to a two-state Boltzmann distribution. E: Activation rise time, determined as the time to 1/2 max following a depolarization to 0 or +20 mV.