Table 2 Primers used for the screening of RNF8, UBC13 and MMS2
From: Mutation screening of the RNF8, UBC13 and MMS2 genes in Northern Finnish breast cancer families
Gene | Exon | Forward primer (5'-3') | Reverse primer (5'-3') | PCR fragment size (bp) |
|---|---|---|---|---|
RNF8 | 1 | GCGAGGAGACCTCTCGAATC | TCCTCTCTGCCATTCATTCA | 498 |
2 | TGCTGCTGGTTGATGAGAT | AAATAAAAGTCATTAGGCTTCTG | 250 | |
3a | AAGAAGACGAAAATCATGAAGC | TAATTCATCCAAACTGAATTTCC | 294 | |
3b | CCTTGTCTTTCCCCAAAGAAT | TTACTTGGCTCAAGGGCAGT | 242 | |
3c | AGTGGCCAGTACACCCTCTG | TTCACATTCATAACGGCTTCA | 240 | |
3d | GGTGACCATGTCCAGGATTC | AAGACCACTCTTGCCCTTCC | 260 | |
4 | CAGGAGATTTTCCACCTGCT | GGTCATGTGATGCCTGTTTG | 271 | |
5 | CAGGCATGTTTGTGGCTAAA | CCTAGCAACCCTTGCACTGT | 242 | |
6 | CCTGTCCCATTTTGCATTTT | AAGGGGTGAGCAACTGTTC | 197 | |
7 | GCCCTTAAGATGGGATTGTTG | TCCCTTTACTCCTCCCCATT | 483 | |
8 | AGGGAAATACAGGCTCCTCA | CAAGTGACTGAGGGCTTCCT | 220 | |
UBC13 | 1 | GACTTCCACTCGTGCGTGA | TCCTCAGCACCCGACTTC | 264 |
2 | TTGGGAGATTGGAGCTGTTC | TGGAATGCTTAAGAGAAAAAGGA | 430 | |
3 | GTCTGTGGGAGGGAAGTGAA | CCCATAGCAAGCCATTTTGT | 385 | |
4 | ATCTTTCAGCCCTGATCCAA | GAGGGGCCACTGCTTTTA | 448 | |
MMS2 | 1 | CCCGGCCCTCATGAACTT | GGTCCCAGGCTACGCTCT | 411 |
2 | AGGGGATTTGGTCTTTTTGG | CACGTGGGAAGCATCAATAA | 421 | |
3 | GCACTTAGACATTAATATTTTAGGTA | TTTTGGCTTAACAAAGGTCCTC | 331 | |
4 | TGCTTAACAAATTGGTGCCATA | GCTGCATTTTTCCTCCTGTT | 408 |