Figure 1

3'UTR plasmid reporter assay. A) Schematic diagram of pTH-GFPa constructs used in mRNA translational efficiency assays. B) 3'UTR plasmid reporter system and FACS analysis (n = 3, duplicate wells analysed in each experiment, 2-tailed t-test, error bars SD); (i) Mean fluorescence intensity (MFI) of RT112 cells transfected with GFP-flanked by mutant 3'UTR sequences relative to that of GFP-flanked by wild-type XPC UTR sequences; (ii) Relative levels of GFP mRNA to 36B4 mRNA analysed by quantitative RT-PCR using the ΔΔCt method. C) Flow cytometry data of individual transfections (n = 3, duplicate wells analysed in each experiment). GFP fluorescence measured on × axis (forward scatter on y axis). See Table 3 for mean fluorescent intensity values.