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Table 1 Comparison of high resolution melting curve analysis and sequencing results for wgaDNA and gDNA samples.

From: Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis

Genes and Amplicons Variants on Melting Curve Analysis (10 patients in each group) Sequence analysis results (10 patients in each group)    
SCN5A gDNA wgaDNA gDNA wgaDNA Type Result Additional information
1 1 5 Wild Type Wild Type    
2 2 2 2 2 G->A pos 87, no aa change  
3 1 1 Wild Type Wild Type    
4 Wild Type 1 Wild Type Wild Type    
5 1 Wild Type Wild Type Wild Type    
6 2 Wild Type Wild Type Wild Type    
7 1 Wild Type Wild Type Wild Type    
8 Wild Type Wild Type Wild Type Wild Type    
9 3 3 1 1 G->A R340Q Rare variant
10 5 6 3 3 C->A Intronic pos -3
11 6 4 3 3 G->A Intronic pos -24
12 7 6 3 3 A->G H556R Known variant
13 3 3 Wild Type Wild Type    
14 Wild Type 4 Wild Type Wild Type    
15 3 4 Wild Type Wild Type    
16 3 3 Wild Type Wild Type    
17 Wild Type Wild Type Wild Type Wild Type    
18 Wild Type Wild Type Wild Type Wild Type    
19 Wild Type 1 Wild Type Wild Type    
20 6 4 3 3 G->A pos 1061, no aa change  
21 Wild Type Wild Type Wild Type Wild Type    
22 1 1 Wild Type Wild Type    
23 Wild Type Wild Type Wild Type Wild Type    
24 Wild Type Wild Type Wild Type Wild Type    
25 Wild Type Wild Type Wild Type Wild Type    
26 3 4 Wild Type Wild Type    
27 1 1 Wild Type Wild Type    
28 2 2 Wild Type Wild Type    
29 2 2 Wild Type Wild Type    
30 Wild Type Wild Type Wild Type Wild Type    
31 1 1 Wild Type Wild Type    
32 Wild Type Wild Type Wild Type Wild Type    
33 7 7 7 7 T->C pos 5457, no aa change 6 heterozygous, 1 homozygous
34 Wild Type Wild Type Wild Type Wild Type    
35 7 7 7 7 A->G Intronic 6 heterozygous, 1 homozygous
KCNA5 gDNA wgaDNA gDNA wgaDNA Type Result Additional information
36 1 1 1 1 C->T pos 381, no aa change Same patient harbouring two variants
      A->G T155C Same patient harbouring two variants
37 1 1 Wild Type Wild Type    
38 Wild Type Wild Type Wild Type Wild Type    
39 1 1 1 1 G->A R578K Rare variant
40 10 10 Wild Type Wild Type    
Total 81 85 31 31