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Figure 3 | BMC Medical Genetics

Figure 3

From: Mismatched single stranded antisense oligonucleotides can induce efficient dystrophin splice switching

Figure 3

Confirmation of the patient dystrophin mutation and the potential effect on AO Annealing. The dystrophin (c.3385 Insertion A) mutation was confirmed by direct sequencing (A). This mutation occurs within the annealing site of H25A(+95+119) suggesting a number of potential scenarios for the binding of H25A(+95+119) or H25A(+95+A+119) including; perfect complementarity between H25A(+95+119) and normal dystrophin mRNA (B), a single base "bulge" in the mRNA when H25A(+95+119) binds to patient mRNA (C), perfect complementarity between H25A(+95+A+119) and patient mRNA (D) and a single base "bulge" in H25A(+95+A+119) when this oligomer binds to normal mRNA (E).

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BMC Medical Genetics

ISSN: 1471-2350

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