Table 1 Primers used for amplification of the ABCB1 and CYP2C19 polymorphisms
Name | Primer sequence | Fragment size (bp) | Anelling temperature (°C) |
|---|---|---|---|
ABCB1 c.C3435T F | 5' GGGTGGTGTCACAGGAAGAG3' | 74 | 59.6 |
ABCB1 c.C3435T R | 5' CCTTCATCGAGTCACTGCCT 3' | ||
CYP2C19*2 F | 5' TGCAATAATTTTCCCACTATCA 3' | 83 | 50.5 |
CYP2C19*2 R | 5' CCTTGCTTTTATGGAAAGTGA 3' | ||
CYP2C19*3 F | 5' CCTTGCTTTTATGGAAAGTGA 3' | 75 | 50.5 |
CYP2C19*3 R | 5' TTTTTGCTTCCTGAGAAACCA 3' | ||
CYP2C19*4 F | 5' GCAAGCTCACGGTTGTCTTA 3' | 70 | 63.4 |
CYP2C19*4 R | 5' TGTGGTCCTTGTGCTCTGTC 3' | ||
CYP2C19*5 F | 5' TGGAAGTTGTTTTGTTTTGCT 3' | 110 | 59.6 |
CYP2C19*5 R | 5' TAAGAGATAATGCGCCACGA 3' | ||
CYP2C19*17 F | 5' AAATTTGTGTCTTCTGTTCTCAAA 3' | 100 | 56.7 |
CYP2C19*17 R | 5' AATCCCAGTTCTGCCAGCTA 3' |