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Table 2 Primer sequences used for cloning DNA constructs.

From: Promoter polymorphism -119C/G in MYG1 (C12orf10) gene is related to vitiligo susceptibility and Arg4Gln affects mitochondrial entrance of Myg1

No Name Sequence
1. Human MYG1 4Arg ATG Fw 5'-ATATgctagcCATGGGACACCGCTTCCTGCGCG-3'
2. Human MYG1 4Gln ATG Fw 5'-ATATgctagcCATGGGACACCAATTCCTGCGCG-3'
3. Human MYG1 noSTOP Rev 5'-ATATccgcggAGATTTGTGGGAGGTATGAG-3'
4. MYG1-300KpnIF 5'-TATAggtaccAGAATGTTGGTCTTTTCTTGGATTAAGC-3'
5. MYG1-1kbKpnIF 5'-TATAggtaccGAGAAGAGTCTCATTCTCACC-3'
6. MYG15utrHindIIIR 5'-ATATaagcttAAGCAGCTCCCTGCAGGGAG-3'
  1. First (ATG) and fourth (CAA or CGC) amino acids in primers 1 and 2 are underlined; restriction sites are given in lowercase letters.