Table 1 Biological characteristics of the genotyped TLR2 polymorphisms and the primers and conditions used for PCR

-100 (GT)n

rs34692294

Forward:

ionhs5'-GCATTGCTGAATGTATCAGGGA-3'

Reverse:

5'-CCACAAAGTATGTGCCATGGTCCAGTGCTTC-3'

Condition: 95°C, 3 min; (95°C, 30 sec; 55°C, 30 sec; 72°C, 1 min) × 35 cycles; 60°C, 60 min

-16934 A>T

rs4696480

Forward: 5'-TGGTTCTGGAGTCTGGGAAG-3'

Reverse: 5'-ACAGAACGGTCTCCAAGTAG-3'

Condition: 94°C, 5 min; (94.1°C, 1 min; 59.3°C, 1 min; 72.2°C, 2 min)× 35 cycles; 72°C, 10 min.

-15607 A>G

rs1898830

Forward: 5'-GCAGCTGAAATCACAGAGCA

Reverse: 5'-AGGATAATGGCCTCCTGCT

Condition: 94°C, 5 min; (94°C,1 min; 67.1°C,40 sec; 72°C,2 min) × 30 cycle; 72°C, 10 min

-196 to -174

Ins>del

not available

Forward: 5'-cacggaggcagcgagaaa

Reverse: 5'-ctgggccgtgcaaagaag

Condition: 94°C, 5 min; (94°C,1 min; 64.5°C,1 min; 72°C,2 min) × 35 cycle; 72°C, 10 min

1350 T>C

(S450S)

rs3804100

Forward: 5'-AACCGGAGAGACTTTGCTCA

Reverse: 5'-AGTTATTGCCACCAGCTTCC

Condition: 94°C, 5 min; (94°C,40 sec; 62°C,40 sec; 72°C,1 min) × 30 cycle; 72°C, 10 min