Table 1 Primers selected for Exons #3 and #7 of Gene ER-β
Polymorphism site | Melting temperature (°C) | Oligonucleotic sequences | Sequence size |
|---|---|---|---|
Exon 3 | 59.23 59.86 | Primers for PCR reaction F TTGCTCCCTAGAGAGACACTGA R CTTCACACGACCAGACTCCA | 151 |
Exon 7 | 60.04 60.14 | Primers for PCR reaction F 5' GATGAGGGGAAATGCGTAGA 3' R 5' GGCCCAGCTGTGTGATTACT 3' | 156 |