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Table 3 Results of the mutation analyses of KIT exon 11 performed in trial 3.

From: Pitfalls in mutational testing and reporting of common KIT and PDGFRA mutations in gastrointestinal stromal tumors

 

Lab A

Lab C

Lab D

Lab E

Lab F

Sample

T3.1

T3.2

T3.3

T3.1

T3.2

T3.3

T3.1

T3.2

T3.3

T3.1

T3.2

T3.3

T3.1

T3.2

T3.3

Experimental Step

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

A

S

Primer Lab A/B

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+/-

+

+

+

+

+

+

Primer Lab C/D

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+/-

+

+

+

+

+

+

Primer Lab E

+/-

-

+/-

-

+/-

-

-

-

-

-

-

-

+

+

+

-

+

+/-

+

+

+

+

+

+/-

+

+/-

+

+/-

+

+/-

Primer Lab F

+/-

-

+

+

+

+/-

+

+

+

+

+

+

+

-

+

+

+

-

+

-

+

+

+

-

+

-

+

+

+

+

  1. For each lab the result of amplification (A) and sequence analysis (S) is detailed. "+" means success of amplification or nominal result in sequence analysis, "+/-" means only faint amplification with unspecific extra-bands or different/not-scorable sequence-analysis, and "-" means failure of amplification or sequence analysis. Primer pairs used in Lab A and B and Lab C and D were the same, respectively. Lab B did not participate in the third trial suppl.