Table 2 Primer sequences and DHPLC analysis conditions for two sets of designed primers.
Exon | Primer sequence (5'-3') | Ampli. size(bp) | PCR anneal. temp.°C | DHPLC Oven temp.°C | Start % buffer B |
|---|---|---|---|---|---|
N1Ex6-1 | F 5' AGCAGGTATACCCAGCAGCTGC 3' R 5' CCCGCTCTGGGTAGCAGTAGTG 3' | 412 | 65.3 | 63.5 | 59-57 |
N1Ex6-2 | F 5'CTACTGCTA CCAGAGCGGGA 3' R 5'CCGGAAGATGATCCAGCAGAA 3' | 427 | 55 | 64.5 | 59-57 |