Figure 3From: Functional analysis of the C-reactive protein (CRP) gene -717A>G polymorphism associated with coronary heart disease Supershift assays confirmed the identification of the DNA-binding protein. Supershift assays with biotin-labeled 25 bp oligonuceotides containing the A allele of rs2794521 and nuclear extracts from HepG2 cells in the presence of anti-GR (Lane 3) or normal IgG (Lane 4). Labeled oligonucleotides incubated without the nuclear extracts were included as negative controls (Lane 1). 2 μg of anti-GR antibody or normal IgG was preincubated with the nuclear extract for 1 hour at 4°C before the labeled probe was added. The experiments were repeated three times with similar results.Back to article page