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Table 2 Different loci selected within the vitamin D receptor sequence and their corresponding primers used during PCR-RFLP method.

From: Association study between vitamin D receptor gene polymorphisms and asthma in the chinese han population: a case-control study

Location Locus Alleles PCR primer PCR Product (bp) Restriction enzyme RFLP products(bp)
Exon 2 rs2228570 C/T F: 5'-CCTGGCACTGACTCTGGCTCTG-3'
R: 5'-GGCTCCCTTCATGGA AACACCT-3'
270 FokI 207/63
Intron 2 rs3782905 C/G F: 5'-AAGACATGGTGTCTGCTTCA-3'
R: 5'-GGTTAGATCGATATGTTTGA-3'
304 DdeI 223/81
Intron 8 rs1544410 G/A F: 5'-GGTGGGACTGAAGAAGCTGAAC-3'
R: 5'-CTTTGGACCTCATCACCGACAT-3'
613 BsmI 357/256
Intron 8 rs7975232 A/C F: 5'-GTAGAATAGAAGGAGGGAAGC-3'
R:5'-AGAGGCAGCGGTACTGCTTGGAGTG-3'
662 ApaI 424/238
Exon 9 rs731236 T/C F: 5'-GTAGAATAGAAGGAGGGAAGC-3'
R: 5'-AGCTTCATGCTGCACTCAGGCT-3'
690 TaqαI 504/186