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Table 1 Information of the primers and PCR-RFLP Analysis

From: Association study of SHANK3 gene polymorphisms with autism in Chinese Han population

SNP

Primer sequence (5'→3')

Product (bp)

RFLP

Allele (bp)

 

rs9616915

Forward: acctgggggcttcacctgacta

Reverse: cccaacccagcacacagagg

207

Ava II

T

(180/27)

C

(121/59/27)

rs13057681

Forward: ccgcaagagccccctggtga

Reverse: caggaggcgctcgtcgatggag

328

Sty I

G

(198/130)

C

(328)

rs6010065

Forward: ccttacctgggtgggcatt

Reverse: acggggagggctcttgtg

423

Hinf I

G

(166/257)

C

(423)

rs2106112

Forward: ctgagccactcggaggttgct

Reverse: gtccgaccttcaccacgttcac

340

   

rs2301584, rs41281537, rs756638

Forward: cgccaacagtccaggtcac

Reverse: cagaaggcatgggctgagtt

489

   

G insertion in exon21

Forward: cgggaggagcggaagtcac

Reverse: ggaccccgttggcaaactct

409

   

A962G in exon8

Forward: cgcacgccatgtgtgcattcct

Reverse: tctcggggttggggggtcagac

473

   

splice doner site of intron19

Forward: gcctggggtggggtctgag

Reverse: ttggagcctgggctgtgtg

754

   
  1. PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism; SNP, single nucleotide polymorphism.