Differentially expressed alternatively spliced genes in Malignant Pleural Mesothelioma identified using massively parallel transcriptome sequencing

Table 1 Normalized EJEI and corresponding RT-PCR results for the top ten differentially expressed exon junctions*

				Normalized EJEI						RT-PCR EJEI result
Normal/Tumor	Gene	Typical AceView sequence	Exon junction location in typical AceView sequence	Meso 1	Meso 2	Meso 3	Meso 4	normal lung	Normalized Meso and Normal EJEI difference	Meso 1	Meso 2	Meso 3	Meso 4	normal lung
normal	EMP2	EMP2.shed3.aAug05	1003	0.000	0.000	0.000	0.000	1.000	1.000	0.079	0.065	0.069	0.071	0.069
normal	CYFIP1	CYFIP1.fAug05	49	0.000	0.000	0.000	0.000	1.000	1.000	0.034	0.056	0.043	0.044	0.052
normal	ACTG2	ACTG2.aAug05	574	0.062	0.000	0.000	0.000	0.938	0.876	0.026	0.054	0.029	0.079	0.209
tumor	C1QA and C1QG	C1QAandC1QG.aAug05	1449	0.221	0.264	0.229	0.286	0.000	0.221	0.139	0.115	0.220	0.190	0.148
tumor	MRPL51	MRPL51.bAug05	542	0.228	0.301	0.215	0.256	0.000	0.215	0.678	0.908	0.710	0.664	0.693
tumor	TXNRD1	TXNRD1.aAug05	1333	0.198	0.220	0.363	0.219	0.000	0.198	0.570	0.507	0.520	0.546	0.461
tumor	CDK4	CDK4.aAug05	1246	0.267	0.223	0.196	0.314	0.000	0.196	2.014	1.495	1.454	1.395	1.248
tumor	hfl-B5	hfl-B5.aAug05	1123	0.225	0.226	0.252	0.256	0.040	0.185	0.897	0.772	0.835	0.654	0.686
tumor	COL3A1	COL3A1.aAug05	1680	0.184	0.363	0.195	0.258	0.000	0.184	0.228	0.336	0.230	0.183	0.168
tumor	DNAJB11	DNAJB11.aAug05	1612	0.265	0.281	0.271	0.183	0.000	0.183	1.094	1.165	1.316	1.280	1.286

ISSN: 1471-2350