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Figure 1 | BMC Medical Genetics

Figure 1

From: Genetic effect of CysLTR2 polymorphisms on its mRNA synthesis and stabilization

Figure 1

Flow cytometric analysis of CysLTR2 expression on B cell lines between haplotype 1 homozygote ( ht1 +/+ , GCAA: c .-- 819G>T , c.2078C>T , c.2534A>G and c. 2545+297A>G ) and haplotype 2 homozygote ( ht2 +/+ , TTGG ). (A) Histogram of CysLTR2 protein expression on the EBV-infected B cell line. Immunofluorescence staining was performed using anti-human CysLTR2 Ab and FITC-conjugated secondary Ab as described in the Methods. The histogram is a representative of 17 independent experiments. (B) Ratio of CysLTR2 positive cells of the EBV-infected B cell lines having ht1 +/+ (n = 8) or ht2 +/+ (n = 9). (C) Mean fluorescence intensity of CysLTR2 expression of the same B cell lines. The ratio of mean FL1 channel values was determined as the FL1 level of CysLTR2 staining to that of isotype-matched control staining. P values were calculated by a Mann--Whitney U-test.

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