Figure 2
From: Identification of a novel functional deletion variant in the 5'-UTR of the DJ-1 gene
The DJ-1 16 bp deletion variant exhibited significantly reduced transcription levels compared to the wild-type (P < 0.0001) in two different cell lines. Box plots of dual-luciferase assays of the wild-type and deletion variant using (A) HEK293 cells and (B) neuroblastoma M17 cells. They include median (horizontal line within box), inter-quartile interval i.e. 25th to 75th percentile (box) and the range of variation (whiskers). The 2 kb promoter fragments (containing either wild-type sequence or the deletion) were linked to the luciferase gene and the constructs were transfected into two mammalian cell lines. For each experiment, firefly luciferase activity was divided by renilla activity to normalize for transfection efficiency. Data are representative of four transfection experiments (triplicate points obtained over four independent experiments). WT: wild-type; D: deletion variant; RLU: relative light units.